Invertase immobilised on montmorillonite: reusability enhancement and reduction in leaching
نویسندگان
چکیده
Invertase was immobilised on microporous montmorillonite K-10 via adsorption and covalent binding. The immobilised enzymes were tested for sucrose hydrolysis activity in a batch reactor. Km for immobilised systems was greater than free enzyme. The immobilised forms could be reused for 15 continuous cycles without any loss in activity. After 25 cycles, 85% initial activity was retained. A study on leaching of enzymes showed that 100% enzyme was retained even after 15 cycles of reuse. Leaching increased with reaction temperature. Covalent binding resisted leaching even at temperatures of 70 C. 2004 Published by Elsevier B.V.
منابع مشابه
A novel system for reducing leaching from formulations of anionic herbicides: clay-liposomes.
A new approach was developed for reducing leaching of herbicides and contamination of groundwater. Liposome-clay formulations of the anionic herbicides sulfometuron and sulfosulfuron were designed for slow release by incorporating the herbicide in positively charged vesicles of didodecyldimethylammonium (DDAB), which were adsorbed on the negatively charged clay, montmorillonite. Freeze fracture...
متن کاملPii: S0038-0717(00)00167-x
The preparation and characterisation of synthetic enzyme complexes simulating those usually encountered in soil were investigated. Complexes were prepared at 308C by the interaction of acid phosphatase and tannic acid in the presence and absence of Fe and Mn ions (as chlorides) and oxides, with and without montmorillonite. In comparison with phosphatase–tannic acid complexes, the enzymatic comp...
متن کاملA monolith immobilised iridium Cp* catalyst for hydrogen transfer reactions under flow conditions.
An immobilised iridium hydrogen transfer catalyst has been developed for use in flow based processing by incorporation of a ligand into a porous polymeric monolithic flow reactor. The monolithic construct has been used for several redox reductions demonstrating excellent recyclability, good turnover numbers and high chemical stability giving negligible metal leaching over extended periods of use.
متن کاملIn vitro carbohydrate stress: salicylic acid increases soluble invertase activity in Pistacia vera L. in vitro plantlets. Françoise Bernard *, Majid Baghai and Shirin Hadad Kaveh
The action of salicylic acid (SA) has been well investigated in plant resistance against pathogen attacks but its role may be extended to a more global anti-stress plant cell strategy. The expression of defense-related functions may be also enhanced by elevated hexose levels. To verify if there exists a relation between these two defense programs, SA effect on soluble acid invertase (EC 3.2.1.2...
متن کاملEnhanced pH and thermal stabilities of invertase immobilized on montmorillonite K-10
Invertase was adsorbed onto micro-porous acid-activated montmorillonite clay (K-10) by two procedures, namely adsorption and covalent binding. The immobilized enzymes were characterized by XRD, surface area measurements and Al NMR. XRD measurements revealed an expansion of clay layers due to immobilization which suggests that intercalation had taken place. Surface area measurements also support...
متن کامل